Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Tris base has been used:

• as a component of H buffer (cell dissociation buffer)

• for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique.

• as an assay buffer for reconstitution of extracted and dried protein samples.

• to prepare Tris-HCl buffer that is used to stabilize proteins.

• as a buffer to extract carotenoid from tubers.

• as a component of sample buffer during protein extraction prior to western blotting[12]

• as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)

• to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay.

• as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate.

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